The morfological and molecular identification of Fusarium verticillioides causing fusariosis on wheat grain

During the 2014-2017 period, wheat samples were collected from discoloured spikes affected by Fusarium head blight (FHB) 20 locations in Serbia. After isolation, fungi cultivated on potato dextrose agar (PDA) at 25oC for 7 days. Based situ identification PDA, 36 isolates of section Liseola selected further analyses. The pathogenicity all was confirmed leaves. virulence determined measuring lengths spots formed inoculation leaf site. In order to prove presence species verticillioides, a pair primers FV-F2/FV-R used. This amplifies sequence gaoB gene, and it proved be specific stated species. Moreover, same purpose, VER1-VER2 based calmodulin partial gene reference isolate RBG 1603 Q27 used as positive control. produced band expected size - 578 bp 18 isolates, while using FV-F2/FV-R, 370bp long F. verticillioides 16 samples. Sixteen out verified with also identified FV-FS/FV-R. No amplification observed negative